FastQCFastQC Report
Fri 29 Apr 2016
ERR1042582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20784428
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239530.11524493240805088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130650.026.1251451
GTATCAA203100.016.5962582
TCTAGCG17950.014.11977828
ACGGACC32800.014.1006098
GACGGAC32250.013.7674427
TCGTTTA26450.013.429111530
CTAGCGG19150.013.04177629
CGAACGA20750.012.48192716
CTAGTAC14250.012.4631583
CGGACCA38150.012.4626489
AAGACGG40900.012.4388755
TACCGTC28650.012.3979057
TACGACG24500.012.3836745
GTATTAG32150.012.3141531
ACGAACG21650.012.13394915
CGCAATA21000.012.06904836
CGCAAGA41850.012.06812
GCGTTAT27350.012.0402191
CAAGACG46100.012.0390454
ATCAACG284050.011.9317024
Produced by FastQC (version 0.11.5)