FastQCFastQC Report
Fri 29 Apr 2016
ERR1042581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11167171
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140140.12549283968159886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114800.10280132721169936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62550.025.7905671
GTATCAA97100.016.61382
TAGACAG21150.013.9078025
ATAACGC11650.013.1802583
TAACGCC10050.012.5174124
TCTAGAC12600.012.4801583
TACCGTC8500.012.4058837
TCAACGC133650.012.2225975
ATCAACG132900.012.22197154
TATACTG28850.012.1837085
TCGTTTA9750.012.1435930
TATCAAC136500.012.1164843
ATACCGT10900.012.0504596
CAACGCA136950.011.9280756
ACGGACC13700.011.7481768
GTATTAG17650.011.7393771
TCTATAC16450.011.6960483
CGTCGTA8100.011.64814910
GTATACG3500.011.62857151
TATACCG4650.011.5376345
Produced by FastQC (version 0.11.5)