FastQCFastQC Report
Fri 29 Apr 2016
ERR1042580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13280980
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.021.1556431
GTATCAA86400.015.0098372
GACGGAC22350.014.0715887
ACGGACC23000.013.9152178
CTAGCGG13700.013.90875929
TCTAGCG13550.013.65313728
CGCAATA14250.013.63157836
TCTATAC12550.013.26693253
TCGTTTA15150.013.18811830
TATACTG23300.013.1802575
TATACAG21200.013.1768865
CTAGTAC8000.012.9500013
ATAACGC18150.012.8429753
CGAACGA11800.012.69915316
TACGACG15600.012.6891035
CGTTTAT16950.012.44247831
TAACGCC17900.012.2988834
TAATACT18700.012.0695184
AAGACGG30200.011.9453635
GTATACG4200.011.8928571
Produced by FastQC (version 0.11.5)