FastQCFastQC Report
Fri 29 Apr 2016
ERR1042578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9982801
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241010.24142522724834442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218760.21913689354320495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT147010.1472632781120249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70300.023.9736841
GTATCAA99550.016.7995992
TATACTG21650.015.0392615
ACGGACC13250.014.1018878
GACGGAC12350.013.9311737
TCTATAC14350.013.6655063
CTACACT23400.013.4401714
TAGACAG18450.012.9349595
CGAACGA3600.012.84722216
TAATACT20750.012.6602414
ATACTGT30550.012.5351886
TCAACGC135450.012.4835735
ATCAACG135700.012.4605754
GTATTAG17000.012.2970591
CAACGCA137100.012.2928516
TATCAAC140600.012.0789473
TAGACTG12850.011.9494165
CTAGTAC6050.011.9256193
AAGACGG17400.011.9080475
AACGCAG143550.011.9080467
Produced by FastQC (version 0.11.5)