FastQCFastQC Report
Fri 29 Apr 2016
ERR1042576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11001208
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140480.12769506766893235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124830.11346935718332023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.021.6337071
GTATCAA81900.015.2246642
TATACTG25850.015.0290135
CTAGCGG7950.014.66037829
TCTAGCG8100.014.38888928
CGAACGA5850.013.91453116
GACGGAC13850.013.8916977
ACGAACG6050.013.7603315
TAGACAG21750.013.52413755
TACCGTC10600.013.2641527
GTACCGT4050.013.2469146
ACGGACC15300.012.6960788
CGTCGTA8600.012.6918610
TCTAGAC13150.012.6615963
ATACCGT13400.012.5634336
TAGACCG2805.456968E-1212.5535725
CTAGACA18750.012.3333334
CGTTATT10900.012.2201832
TCTATAC16050.012.2180693
CGCAATA10800.012.16203636
Produced by FastQC (version 0.11.5)