FastQCFastQC Report
Fri 29 Apr 2016
ERR1042575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425187
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44600.1839033443606617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43080.17763578643626243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2350.019.680851
GGTATCA20450.019.0880181
TATACTG4050.018.2716055
ATACCGT3650.015.7123296
GCTAACG957.0648406E-515.57894826
TAGTACT3450.015.5507244
GTATCAA25750.015.0155342
ATGCGAC1853.0559022E-1015.021
TACCGTC2109.094947E-1214.97619157
CTAGGCA4600.014.8804354
TGCGACG1904.5656634E-1014.60526422
TACGACG1702.4403562E-814.1470595
TAATAGT2750.014.1272744
TACTAGG2900.014.0344832
ACTAGGC2650.013.9622643
TAGACAG5050.013.9207935
GTACGTA800.006301279613.87500113
GTATTAG3350.013.8059691
CTAACGG950.001245770813.63157927
GTGCGAA1102.4581238E-413.45454513
Produced by FastQC (version 0.11.5)