FastQCFastQC Report
Fri 29 Apr 2016
ERR1042574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622159
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138780.20956911484607965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT122140.18444135817336915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87470.13208683150011952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.022.124491
GTATCAA70900.015.1861792
GACGGAC7350.014.8503417
TATACTG11500.014.3173915
ATAACGC7450.014.1543623
ACGCGCG5700.013.9561421
ATACCGT8750.013.9542866
ACGGACC8300.013.8192778
GTATAGA11250.013.8133331
GTATTAG15850.013.7728721
TAACGCC6650.013.6315784
GTATACG3800.013.63157751
TACCGTC6550.013.5572517
CTAGTAC4650.013.5268833
TTAGACT7800.013.519234
TCTATAC9350.013.4545463
CGCGCTA5950.013.36974824
GTACTAG5400.013.3611121
GTAAGAC6850.013.2335783
TAATACT16700.013.1826344
Produced by FastQC (version 0.11.5)