FastQCFastQC Report
Fri 29 Apr 2016
ERR1042573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470739
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.020.2411771
TCTATAC1700.019.5882363
TATACTG4650.016.3118275
CGTTTAT2100.015.85714231
GTAATAC3150.015.2698413
TCGTTTA2059.276846E-1114.43902430
TAATACG1551.2118107E-714.322584
TAATACC2201.8189894E-1114.2954544
TTACACC3800.014.1184224
TACACGC1051.656847E-414.0952385
GTATCAA11700.013.9145312
CAATCGT800.00630132313.87500114
ATTGACG3200.013.87500132
ACTAGAC2800.013.8753
GACGGAC2950.013.796617
TTACGCT950.001245783113.6315794
GTACGAT950.001245783113.6315796
TATAGAC2457.2759576E-1213.5918363
TAACACA3550.013.5492964
TACACTG9700.013.5412375
Produced by FastQC (version 0.11.5)