FastQCFastQC Report
Fri 29 Apr 2016
ERR1042571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815550
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146180.21448012266068037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117840.1728987389132205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96020.14088371444710993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.023.9514771
GTATCAA64500.015.4596882
TATACTG12150.014.6172855
CGCGTAT1501.3080898E-613.56666712
TAGACTG9550.013.5602095
GTACCGT1805.171387E-813.3611116
CGAACGA3750.013.3216
TCTATAC9350.013.2566853
CTATACT13450.012.5167294
CTAGTAC4950.012.3333343
GACGGAC5700.012.3333347
GTAAACG4500.012.33333327
TTAGACT6500.012.2384624
TATACCG2901.2732926E-1112.1206895
CTACACT18800.012.1037234
GTACTAG4900.012.0816341
ATCAACG82350.012.0637534
TCAACGC82550.012.0569345
GTATTAG12750.012.0431381
TAGACCG1705.3187596E-611.9705895
Produced by FastQC (version 0.11.5)