FastQCFastQC Report
Fri 29 Apr 2016
ERR1042570.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042570.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9711547
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT455260.4687821621004357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT390330.4019236070216208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290520.29914904391648417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169570.1746065791577799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123200.024.5515421
GTATCAA182500.016.5232872
ACGGACC10250.014.7999998
GTACTAG6850.014.5839421
CGCGATA1406.007649E-714.53571414
TATACTG20450.014.38386255
TACCGTC8350.013.73652657
GTATACG4750.013.24210551
CGTCGTA7000.013.21428610
GACGGAC10900.013.0688087
ATTGACG9350.013.05882332
CTAATCG5850.012.96581326
AACGCAG234950.012.8188977
TAACGCC7300.012.6712334
ATCAACG237550.012.6708074
TCAACGC240550.012.5358545
GTATTAG23200.012.4396551
CAACGCA242150.012.4301056
ATAACGC9750.012.3333333
TATCAAC247750.012.3134213
Produced by FastQC (version 0.11.5)