FastQCFastQC Report
Fri 29 Apr 2016
ERR1042569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547587
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7000.1278335679992403No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5480.10007542180511955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC601.335271E-624.6666663
GGTATCA3600.024.6666661
AGCGTTA701.9191066E-723.78571310
GGATACG400.001929902523.1250021
GACGGAC903.8180588E-922.611117
CGGACCA903.8180588E-922.611119
GTATCAA4050.021.9259262
ACGGACC957.1249815E-921.4210538
GAGCGTT806.9464295E-720.81259
CTGCGCG555.1383674E-420.1818189
CGGATAG555.1383674E-420.18181814
GGAGCGT1103.8406142E-818.58
TTAAGCT500.007030293318.54
CGTAGAC609.228399E-418.53
GCTACGA500.007030293318.52
CGTTATG953.5994126E-617.52631612
TTAGGTT752.0647769E-417.2666664
ATTACAC752.0647769E-417.2666663
GTATACT650.001578505517.0769254
TATACTG650.001578505517.0769255
Produced by FastQC (version 0.11.5)