FastQCFastQC Report
Fri 29 Apr 2016
ERR1042568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551110
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146090.170843317417271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125470.1467294889201519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102730.1201364501216801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.022.2912711
GTATCAA75750.015.3617162
TATACTG18950.015.0343015
TCTATAC12350.014.9797563
ACGGACC9800.014.9132658
TAACGCC6600.014.5757574
GACGGAC9900.014.0151527
TAATACT14900.013.5335574
TTACACT16150.013.1733754
CTAATAC15400.013.0941573
GTATTAG15200.013.0230261
ATACCGC9100.013.01098827
ATAACGC8250.013.0060613
CTATACT17150.012.9446074
ACGAACG3750.012.82666815
GTACATG152300.012.6694021
CTAGCGG5850.012.64957229
CTAGACA12100.012.53718954
TTAATAC13350.012.47190953
TCTAGCG5800.012.43965628
Produced by FastQC (version 0.11.5)