FastQCFastQC Report
Fri 29 Apr 2016
ERR1042565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584073
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.025.4374981
TAGACCG400.00193150823.1255
GTATCAA10050.020.0646762
CGTATAC500.00703602918.4999983
TGTATAC1800.017.4722213
CGCGTAA1800.017.47222110
ACGGACC2500.017.028
TATAGAC1201.04189894E-716.9583343
TAAGACG803.3835758E-416.18754
TAGACTA700.002593074515.8571425
CTAGTAC700.002593074515.8571423
GACGGAC2700.015.7592587
TAGACTG1659.749783E-1015.696975
TACCGCA1659.749783E-1015.6969723
GTAGCAC2600.015.6538463
TCGCGTA1902.7284841E-1115.5789489
TACCGTC1557.214112E-915.51612957
GTTAGAC855.365741E-415.2352943
TCAACGC13600.015.2352945
ATTAGAC1101.4524785E-515.1363643
Produced by FastQC (version 0.11.5)