FastQCFastQC Report
Fri 29 Apr 2016
ERR1042564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578828
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131970.20059803965083142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113980.17325274349777803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102230.15539241944005833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.026.772681
GTATCAA77950.016.067352
TAACGCC4100.014.4390254
ACGGACC6400.013.8750018
TCTATAC9150.013.5464493
AACCCGT5950.013.36974829
CTAGTAC4450.013.303373
CGTAATA1857.400922E-813.02
TAGTACT9050.012.8784534
GTATTAG10750.012.7348831
CGGGTAA4650.012.73118224
CCTATAC7300.012.6712333
GACGGAC6600.012.6136367
CGAACGA5300.012.56603716
CGTCGTA5300.012.56603710
TACCGTC6400.012.4296887
TATACTG14800.012.3755
ATAACGC5000.012.213
TAACCCG5050.012.08910928
CTTACAC10500.011.9809533
Produced by FastQC (version 0.11.5)