FastQCFastQC Report
Fri 29 Apr 2016
ERR1042563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7563573
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91650.1211728901142357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80120.10592877202348677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.024.6966041
GTATCAA65600.015.5388712
TACGACG7000.014.5357145
GACGGAC8700.013.8218397
CTAGTAC6100.013.6475413
TAAGACT10800.013.5324084
CGACGGT7750.013.3677437
GACGGTA7700.013.2142858
ACGACGG7750.013.1290336
CGAACGA6200.013.12903216
GTATTAG12550.012.9721121
ACGGACC9300.012.9301078
TATACTG18650.012.8954425
GTATAGA10400.012.6298071
TATACAG15600.012.5705135
ACGAACG6450.012.33333315
TAGACAG13750.012.2436365
CTATACT11900.012.1260514
TAATACT11450.012.1179044
TAGACTG13450.011.9665425
Produced by FastQC (version 0.11.5)