FastQCFastQC Report
Fri 29 Apr 2016
ERR1042562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8849165
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129000.14577646591514565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103200.11662117273211654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95430.10784068327350659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62350.025.8436261
TCGTTTA13600.016.59558930
GTATCAA101500.015.8024642
GTAAACG14900.015.271811527
CTAACGC1954.1836756E-1115.1794873
TAACGCC16350.015.0489294
ACGGACC17600.014.6107958
CCGGTCG15400.014.53571420
TAAACGC15850.014.35646628
GCGTTAT12600.014.0952391
CGTTTAT16050.014.06230531
ATAACGC16850.014.0534133
AAGACGG20150.014.0471475
TCTAGAC9700.013.9226813
GACGGAC18150.013.8622597
CAAGACG21000.013.8309524
CTAGCGG10200.013.78431329
TCTAGCG10100.013.73762328
GTATTAG18450.013.5365861
AATAACG17350.013.3285292
Produced by FastQC (version 0.11.5)