FastQCFastQC Report
Fri 29 Apr 2016
ERR1042561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9384419
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112490.1198689018467739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58850.025.7145291
GTATCAA92600.016.2024842
TCTAGCG9750.015.17948728
GACGGAC16800.015.0863097
CTAGCGG10050.014.72636829
ACGGACC17150.014.6705548
TCGTTTA11800.014.58050830
AAGACGG20350.014.1818185
CTAGGAC15300.013.9052293
ATAACGC14150.013.8586573
TAACGCC14200.013.5492964
GTAAACG11700.013.4401727
CGCAATA11300.013.42477836
AGACGGA20600.013.3810686
CGCAAGA21250.013.2329422
TCTATAC11050.013.2262453
TAATACT16100.013.099384
CGGACCA19650.013.08651359
CCGGTCG13750.013.05090920
GCGTTAT11250.012.9911121
Produced by FastQC (version 0.11.5)