FastQCFastQC Report
Fri 29 Apr 2016
ERR1042560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10079695
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.023.7604941
GTATCAA82850.015.0054322
TCGTTTA12300.014.43902530
TCTAGCG11500.014.31739128
CGCAATA11950.014.08786736
ACGGACC19450.014.0771218
GCGTTAT15750.013.97777751
CTAGCGG11850.013.89451629
GACGGAC19200.013.7786467
CGTTATT13350.013.5805242
TTAGACT8000.013.41254
TTAACGG3850.012.97402635
GTAAACG14700.012.83673427
TAAACGC15300.012.81699428
TATACCG3900.012.8076935
ACGAACG9700.012.58762915
TATACTG14300.012.290215
CGTTTAT14150.012.15901131
AAGACGG23550.012.0976655
CGAACGA9950.012.08542716
Produced by FastQC (version 0.11.5)