FastQCFastQC Report
Fri 29 Apr 2016
ERR1042559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5485807
Sequences flagged as poor quality0
Sequence length43
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC215730.3932511661456555No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC181160.3302340020347052No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC115740.21098080920455278No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA114650.20899386361933622No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG110200.20088202155125034No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG106870.1948118116441209No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC106150.1934993338263632No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC95110.17337467395407824No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC94820.17284603705525914No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT92220.16810653382446739No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC72360.13190402068465043No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC65380.11918027739583256No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG62080.11316475406444303No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT60240.10981064408572885No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA58870.10731329046027321No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG57170.10421438450167861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.026.1259561
ATTATAC705.107717E-621.1428593
TAGTACT1700.020.6764724
TAATACT1600.019.656254
GTATCAA35350.019.258842
TATTAGG1254.129106E-1019.242
TTAGAAC1502.5283953E-1017.2666663
TATACAA752.0690588E-417.2666662
TTAATAC1401.873559E-917.1785723
TAGACAG5450.016.9724775
CGTTGAA26100.016.94061333
TTTATAC1452.9867806E-916.5862063
AACCCGT27550.016.45190429
ACCCGTT27750.016.430
TTATACT2050.016.2439024
CGTTTAT6400.016.187531
TCGTTTA6500.015.93846130
TAAAAGT700.002594165515.8571434
CTAGTAC1403.480818E-815.8571433
AATTAGA1659.767973E-1015.6969692
Produced by FastQC (version 0.11.5)