FastQCFastQC Report
Fri 29 Apr 2016
ERR1042558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13977385
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT194550.13918912586295648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT152330.1089831896309646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT143130.10240112868036474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99450.023.7737561
GACGGAC23000.016.0065217
TCTAGCG16800.015.52678528
GTATCAA151200.015.514552
ACGGACC23800.015.4684888
CGCAATA17300.015.39884436
CTAGCGG17550.014.86324829
TCGTTTA18400.014.77989230
CAAGACG29650.014.2259694
AAGACGG29550.014.14895
GTAAACG20650.014.06537427
TAAACGC21150.013.557919528
TATACTG29650.013.4772345
AGACGGA29150.013.0737566
CGGACCA27600.013.0036239
TAGACAG25950.012.9036615
CGCAAGA30550.012.8985272
ACGAACG16500.012.78181815
GTATTAG25300.012.5770741
TAACGCC22800.012.5767554
Produced by FastQC (version 0.11.5)