FastQCFastQC Report
Fri 29 Apr 2016
ERR1042556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11819454
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT214010.18106589356834926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190560.16122572159424622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160920.13614842106919658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.027.8633581
GTATCAA134750.016.8181822
ATAACGC18200.013.6208783
TCTATAC16400.013.5365853
TCGTTTA14650.013.38566530
TAACGCC15900.013.3805044
GTATTAG27800.013.37591
ACGCGCG21800.013.32339521
CGCGCTA21500.013.25116324
ACGGACC16250.012.9784618
GACGGAC16300.012.4846627
GTAAACG13800.012.19927627
GTATAGG16400.012.0701221
GCGCGCT23950.012.05010423
TATACTG28450.012.0298775
CGCATCG16050.011.98753913
CGAACGA11800.011.91525516
TTAACGG5000.011.84000135
TCTAGAC14850.011.8350163
ATCAACG193100.011.7361474
Produced by FastQC (version 0.11.5)