FastQCFastQC Report
Fri 29 Apr 2016
ERR1042555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10075622
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156430.15525592365414265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141100.14004098208527474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120760.1198536427825498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.025.7031611
GTATCAA104200.015.8368532
TATACTG23400.014.4679495
TCGTTTA11900.013.83613430
TAACGCC12950.013.5714284
GACGGAC13150.013.5057037
TCTAGCG8650.013.473989528
CGAACGA7950.013.26415116
CTAGCGG8650.013.26011729
ACGAACG8150.013.16564615
ACGGACC14650.012.8805468
ATAACGC14100.012.8581553
TAATACT18900.012.6269854
CCGGTCG12900.012.62015520
GCGTTAT10750.012.5627911
TAACGAA8750.012.47428613
TAGACAG18100.012.2651935
GTATAGA16650.012.2222221
GTAAACG10850.011.93548427
CGCATCG14050.011.850533513
Produced by FastQC (version 0.11.5)