FastQCFastQC Report
Fri 29 Apr 2016
ERR1042554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9201731
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203230.22086061850753952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT171040.18587807011528593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142540.15490563677638478No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA106590.11583690068749021No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC104510.11357645642977392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.026.3137261
GTATCAA148550.016.7502542
TCTAGCG13150.016.31939128
ACGGACC24000.015.956258
GACGGAC24900.015.7510047
CTAGTAC9100.015.6538463
TGCGACG5200.015.65384622
CTAGCGG14050.015.53736729
TAGTACT10650.014.9389674
AAGACGG27600.014.8134065
GTACTAG7750.014.8000011
CGGACCA27000.014.6629639
CGCAAGA28250.014.0141592
CAAGACG31900.013.9184964
TAATACT14700.013.8435374
TATACTG16150.013.746135
TCTATAC9650.013.61143
TAACGCC22550.013.5365864
TAGGTCG5750.013.51304321
GCGTTAT18950.013.4722961
Produced by FastQC (version 0.11.5)