FastQCFastQC Report
Fri 29 Apr 2016
ERR1042553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6396537
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98200.15352056902039338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82560.12906983888313317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64680.10111721389245462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.026.4348851
GTATCAA61700.017.6604542
TCTATAC7650.016.2026143
ACGGACC8350.014.8443118
TATACCG3150.014.6825395
TACCGTC7600.013.8757
ACCCGTT7000.013.74285730
TAATACT12150.013.7037044
TAGACTG10650.013.5492965
ATACCGT9950.013.3869346
TACTAGG8500.013.2764692
TGCGACG2651.8189894E-1213.2641522
GTAAGAC7500.013.0733343
GTACTAG6100.013.0409841
GACGGAC8800.013.0340917
CGAACGA5000.012.95000116
GTGTAAG11750.012.9106391
GTATACG3500.012.6857141
TACGACG5850.012.6495735
ATCAACG88950.012.5413154
Produced by FastQC (version 0.11.5)