FastQCFastQC Report
Fri 29 Apr 2016
ERR1042552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9112890
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188310.20664136185118004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144480.15854465487896813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137690.15109367061382284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74950.026.731821
GTATCAA120400.016.5946852
TCTAGCG9400.015.74468128
TCGTTTA10400.015.29807730
TATACTG16850.014.931755
CGCAATA10850.014.66359336
ACGGACC15150.014.4092428
CTAGCGG10400.014.23076929
GACGGAC14550.013.9862547
GTACTAG6150.013.83739951
GTATTAG19750.012.83291151
ATAACGC15400.012.6136363
CGGACCA17200.012.4767449
GTATAGA13050.012.4750961
TAACGCC14550.012.4604814
CGGTCCA14950.012.25083710
AAGACGG19300.012.1735755
ATCAACG165450.012.1320654
CGTTTAT13300.012.10150431
TATCAAC169250.012.0892173
Produced by FastQC (version 0.11.5)