FastQCFastQC Report
Fri 29 Apr 2016
ERR1042551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8083084
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116670.14433847279083084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96500.11938512577625074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90420.11186324427656573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.025.446641
GTATCAA83100.015.450062
TATACTG17200.014.1976745
TCTATAC12400.013.7258073
GTATTAG18650.012.8954421
CGCGCTA7250.012.75862124
CTATACT16400.012.746954
GTATAGA16000.012.4874991
GTACTAG5900.012.2288131
TTATACT17300.012.1907514
ACGCGCG7650.012.09150321
TGCGTTA3103.6379788E-1211.93548437
ATCAACG108800.011.7665444
TATCAAC111750.011.6876963
TAATACT20500.011.6414634
GACGGAC7000.011.62857157
TCAACGC110250.011.62857155
CTAATAC18950.011.6174143
ACACCGT7150.011.3846166
CAACGCA113050.011.3732866
Produced by FastQC (version 0.11.5)