FastQCFastQC Report
Fri 29 Apr 2016
ERR1042550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8599311
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126250.1468140877798233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110240.1281963171235463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95580.11114843968313276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.026.0412791
GTATCAA77600.016.163662
TATACTG19400.013.6365985
TAACGCC5500.013.4545464
GTATTAG16900.013.2455621
TATACCG5250.012.6857145
CGATTAC2255.7225407E-912.3333331
TCTACGC3500.012.1571443
GTATAGA15600.011.9775651
GTATATA16500.011.7727261
ATCAACG105800.011.7679594
TACACTG27550.011.7513615
CTAACGC3002.3646862E-1111.7166673
TCAACGC107050.011.6823915
TATCAAC109050.011.6547453
CTAGTAC7500.011.5933343
CAACGCA108500.011.5262686
TCTATAC12650.011.4071153
GTACTAG7300.011.404111
CTAATAC14350.011.3449483
Produced by FastQC (version 0.11.5)