FastQCFastQC Report
Fri 29 Apr 2016
ERR1042549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481775
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134360.1417034257826198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113300.1194923946202056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98850.10425263202301256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.025.0353261
GTATCAA90050.015.3053872
CTAGTAC9500.014.83
TATACTG20350.014.0909095
GTACTAG8600.013.5523251
CGCGATA1409.575473E-613.21428614
GTATAGA16550.013.1903321
TACGTAG6850.012.96350414
CTAACGC2951.8189894E-1212.5423733
TAGTACT13300.012.5187974
ACGGACC8550.012.3333338
TCTATAC11350.012.2246693
GTGTTAG15000.011.9633341
TAGACTG14400.011.9479165
CGAACGA5150.011.85436916
TCTAGCG5150.011.85436928
GTATTAG22200.011.7500011
TAGACAG20550.011.7031635
TATATCG5550.011.6666685
ATACTGT26050.011.6468326
Produced by FastQC (version 0.11.5)