FastQCFastQC Report
Fri 29 Apr 2016
ERR1042548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708999
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120940.18026534211735612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.16276645741041249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94370.14066181855147095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49200.025.9827231
TAACGCC7650.016.2026144
GTATCAA79850.016.0557292
TCTATAC7700.015.8571443
ATAACGC8100.015.5308643
CTAGCGG5850.015.17948629
TACCGTC6650.014.4661667
GTATAGA11700.014.3888881
TCGTTTA7400.014.2530
GACGGAC9750.013.8512827
CGGACCA10300.013.6504869
TACGACG7200.013.6180565
TCTAGCG6550.013.55725128
TATACTG13600.013.3308825
ACGGACC10300.013.2912638
AACGCCG8550.013.1988315
GTATTAG15700.012.9617831
ACGACGG7800.012.8076936
AAGACGG12700.012.6732285
CGACGGT7600.012.6578957
Produced by FastQC (version 0.11.5)