FastQCFastQC Report
Fri 29 Apr 2016
ERR1042547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691995
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117400.15262620425520299No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.11748577579678612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88870.11553569652606378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.026.5185181
GTATCAA82250.015.924622
CGTATCG2059.276846E-1114.43902510
TATACTG16400.014.32622055
TCTATAC9950.014.1306533
GTATTAG19000.013.8263171
TATACCG3000.013.5666675
TATCCCG4650.013.1290325
TTAACGG3000.012.9535
ATACTGT23250.012.8903236
TAGACTG11100.012.55
TAATACT15650.012.4121414
GACGGAC10100.012.2722777
ATCAACG108250.012.168134
TAGACAG14750.012.1661015
TATCAAC112000.012.0250013
GTGTAAG13550.012.014761
TCAACGC109750.012.0018225
GTATACG2654.2564352E-1011.8679251
GTAAACG6900.011.79710127
Produced by FastQC (version 0.11.5)