FastQCFastQC Report
Fri 29 Apr 2016
ERR1042546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7338151
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103170.14059399976915166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85780.11689593195888175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79410.10821527112211238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.025.33051
GTATCAA80450.016.6488512
TCTATAC9500.015.3842123
TAACGCC10700.015.0420564
TCGTTTA9500.014.99473930
TAACGGC3000.014.836
ATAACGC11700.014.5470093
TCTAGCG8650.014.32947928
GACGGAC11300.013.7522137
ACGGACC12050.013.3568478
ATACCGT10100.013.1881186
CTAGCGG9000.013.15555629
GTATAGG9900.013.0808091
GTAAACG10400.012.98557727
TAAACGC10550.012.97630428
CGCAATA9500.012.852632536
GTACGAC2753.6379788E-1212.7818183
TATACTG14550.012.7147775
ACGAACG6550.012.70992415
AAGACGG14150.012.6819785
Produced by FastQC (version 0.11.5)