FastQCFastQC Report
Fri 29 Apr 2016
ERR1042545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9468614
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108630.11472640029469995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54800.025.1167891
GTAAACG11600.015.31034527
GTATCAA89300.015.1646142
CTAGTAC6350.014.8582673
TAAACGC12150.014.61728428
TATACTG17050.014.1055725
ACGGACC14350.013.9233458
TCTAGCG8650.013.90173528
GACGGAC14450.013.826997
TCGTTTA10700.012.79439330
CGGACCA15950.012.6426349
AACGCTT16000.012.60312530
CGTTATT9700.012.5876292
CTAGCGG9750.012.52307729
ACGCTTC15900.012.44968531
GTATTAG17350.012.36887551
TACGACG10500.012.3333345
GTACTAG7200.012.3333331
TCTATAC11000.012.2772733
AAGACGG19350.012.2377265
Produced by FastQC (version 0.11.5)