FastQCFastQC Report
Fri 29 Apr 2016
ERR1042544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8494968
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT153580.180789380254287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129610.15257267596534796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116560.13721064046386047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.024.3496861
ATACGCG953.6118818E-617.52631630
GTATCAA100750.015.4977672
TATACTG16650.015.4444455
TTAACGG3450.015.01449335
GTATTAG21700.014.4930881
GACGGAC12850.014.108957
ACGGACC13000.013.9461558
AAGACGG16450.012.933135
GTATAGG12350.012.8825911
ATACTGT24150.012.7163576
GTAAACG9800.012.64795927
TAAACGC10000.012.5828
TAACGCC10100.012.4554464
TACGACG8500.012.4058835
TAACGGC4050.012.33333336
ATAACGC12000.012.3333333
TCTATAC12400.012.2338713
TCGTTTA8800.012.19318230
CGCAAGA15700.012.0191092
Produced by FastQC (version 0.11.5)