FastQCFastQC Report
Fri 29 Apr 2016
ERR1042543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5912009
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109870.18584207162066227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT92710.15681640538774552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83700.14157623914307302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.028.8050941
GTATCAA75450.017.2127252
GTATACG1201.9387062E-615.4166671
CTAACGC1353.9799488E-715.0740753
TATGCCG1252.9635848E-614.79999912
TCGTTTA6900.014.74637730
CGCGTAT800.006302694313.87512
TAACGCC8450.013.79294
ATCGCTC11700.013.12393216
AAATCGC12000.013.10416714
CGAACGA8850.012.96045216
TATACTG12800.012.8632815
TCTACGC2451.1459633E-1012.8367343
ACGCGCG15450.012.81229921
CTAGTAC4200.012.7738093
CTAGCGG5100.012.69607829
ATAACGC8950.012.6089373
CAAATCG12200.012.58606613
CGCGCTA15900.012.56603724
TACCGTC8250.012.5575767
Produced by FastQC (version 0.11.5)