FastQCFastQC Report
Fri 29 Apr 2016
ERR1042542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150594
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72520.11790731106621573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.024.2267511
GTATCAA60350.015.6338032
TCGTTTA8200.014.890244530
GTATTAG11200.014.7008931
GTAAACG8100.014.61728427
TCTAGCG6900.014.47826128
TATACTG10750.014.4558135
AAGACGG14250.014.4105265
GACGGAC13100.014.404587
TAAACGC8150.014.30061428
ACGGACC13400.014.0820898
CAAGACG16400.013.9878054
TATACCG2252.7284841E-1113.97777755
CGCAAGA14450.013.9550182
TACCGTC10700.013.6588787
CGCAATA8050.013.32919336
CTAGCGG7650.013.30065329
TAACGCC9550.013.1727744
CGGACCA14450.012.8027699
CGCGCTA13900.012.64388524
Produced by FastQC (version 0.11.5)