FastQCFastQC Report
Fri 29 Apr 2016
ERR1042541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6214673
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77560.12480141754843738No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC71010.11426184450895485No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA67500.10861392063588865No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT62880.10117990117903226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.026.8331241
TCGTTTA10050.017.48756230
ACGGACC16450.017.2066888
GTATCAA62700.016.965712
GACGGAC16200.016.9012347
CAAGACG18300.016.4781444
CGCAAGA18150.016.1046852
CGGACCA17650.016.0368279
AAGACGG18600.016.0134415
CGTTTAT11300.015.71681331
CCGGTCG11900.014.92437120
TAACGCC12700.014.8582684
GCGCAAG20400.014.6911761
CTAGCGG7700.014.65584629
AGACGGA19700.014.5558386
TACCGTC11000.014.2954547
ATCGTTT12750.014.21960729
ATAACGC13600.014.1470593
ATACCGT12700.014.1299216
GCGAAAG19750.013.95696318
Produced by FastQC (version 0.11.5)