FastQCFastQC Report
Fri 29 Apr 2016
ERR1042540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914292
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76650.12960131153483798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.10773901592954829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38700.022.993541
TCTAGCG7300.017.23287628
CTAGCGG8000.015.72529
GTATCAA56350.015.6273292
GACGGAC12450.015.4538157
TACGACG9950.015.0603025
GTATTAG12550.014.8884461
ACGGACC13100.014.8282438
CAAGACG15400.014.6558444
CGACGGT10100.014.6534667
AAGACGG14550.014.6219925
ACGACGG10450.014.162686
CGCAATA9550.014.14136136
GACGGTA10550.013.853088
CGGACCA14650.013.7645049
TATACTG9950.013.5728655
TACCGTC9000.013.5666677
AGACGGA15500.013.4870966
CGGTCCA10300.013.47087310
ACGGTAT11000.013.4545469
Produced by FastQC (version 0.11.5)