FastQCFastQC Report
Fri 29 Apr 2016
ERR1042539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6768867
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108740.16064726932882564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79920.11806998128342601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70330.10390217447026216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.023.2747441
GTACTAG5050.015.7524761
GTATCAA79600.015.7110552
CTAGCGG8950.014.26256829
TCGTTTA10300.014.1893230
GTAAACG12700.014.12992127
TAAACGC13000.013.94615428
TCTAGCG9450.013.70370428
TTAACGG3000.013.56666735
TATACTG11800.013.483055
TACGACG9650.013.4196895
TAACGCC12100.013.3016534
ACGAACG5800.013.07758615
CGCTTCG14600.013.0513732
GACGGAC13700.012.9635047
TTCGGGC14350.012.89198635
TTAGACT7500.012.8266664
ACGGACC13850.012.8231058
GTATAGA9100.012.8076921
CGAACGA6100.012.73770516
Produced by FastQC (version 0.11.5)