FastQCFastQC Report
Fri 29 Apr 2016
ERR1042538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5863569
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67250.11469124009626219No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC60490.10316242547840744No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58930.10050192979736404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.025.4548871
AAGACGG16850.017.237395
TCGTTTA10800.016.78703730
GTATCAA60850.016.4478232
ACGGACC15900.016.405668
GACGGAC15900.016.1729557
CGTTTAT11800.015.83474531
AGACGGA18200.015.2472526
CAAGACG18850.015.11405854
GTACCGT2109.094947E-1214.976196
CGGACCA17600.014.9261369
TATACTG8650.014.5433525
TAACGCC13850.014.4259944
ATAACGC13950.014.1899643
CGCAAGA18900.014.0952392
TACGACG10000.014.0599995
GCGCAAG19050.013.9842521
TGGTCGG13300.013.90977437
GCGAAAG18800.013.77659518
ATGGTCG12700.013.69291336
Produced by FastQC (version 0.11.5)