FastQCFastQC Report
Fri 29 Apr 2016
ERR1042536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9090984
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156900.17258857787011836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117400.12913893589516823No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT113490.1248379713351162No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC111380.12251699045999861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106850.11753403151958028No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA101270.11139608209628353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109450.018.1027871
TCGTTTA20850.017.2134330
AAGACGG26950.016.7495375
GACGGAC26650.016.1744847
TCTAGCG16150.016.03715128
CAAGACG28750.015.8939134
CGCAAGA28150.015.7726472
ACGGACC27850.015.6104138
TAACGCC25250.015.2396044
CTAGCGG17250.015.01449229
ATAACGC26250.014.9409523
CGTTTAT24250.014.87628831
AGACGGA30100.014.750836
CCGGTCG24450.014.67893720
CGAACGA18000.014.59444416
CGCAATA17900.014.57262636
GCGCAAG31550.014.5419971
CGCATCG25050.014.40119813
ATGGTCG23400.014.38888836
AATAACG26000.014.2307692
Produced by FastQC (version 0.11.5)