FastQCFastQC Report
Fri 29 Apr 2016
ERR1042535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303157
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT141990.152625608704658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112480.12090519379604149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103010.11072585360001987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.024.7551571
GTATCAA88450.015.4149242
TACCGTC9750.014.6102567
CGTCGTA8550.014.49707610
GACGGAC12950.013.5714287
TATACTG16950.013.5339225
ACCGTCG9300.013.5268818
ATACCGT12900.013.4806196
GTATTAG25150.013.4612331
CCGTCGT9750.013.2820519
ACGGACC13450.013.2044618
CTAGTAC8150.013.1656443
TCTAGCG7600.013.14473728
TAACGGC4850.012.96907236
TTAGACT11550.012.8138534
AAGACGG16850.012.7359055
CTAGCGG8000.012.71874929
GTACTAG7250.012.50344851
CTAATAC28050.012.2014263
TAATACT25450.012.1394884
Produced by FastQC (version 0.11.5)