FastQCFastQC Report
Fri 29 Apr 2016
ERR1042534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688371
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91050.1361318024972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74790.1118209501237297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.023.8178141
GACGGAC14150.018.0424027
ACGGACC14050.018.0391468
AAGACGG16700.016.8383245
CGCAAGA17350.015.7809812
AGACGGA16950.015.607676
GTATCAA56850.015.3597192
CGGACCA16950.015.2802369
TCGTTTA8800.014.71590930
CGCATCG9500.014.60526313
GCGAAAG17650.014.35977318
CAAGACG19750.014.3316464
TATACTG13250.014.1018885
TACCGTC8550.014.0643277
GCGCAAG20150.013.9553361
TAACGCC10300.013.8300964
CGCGCTA9350.013.45454624
TAGACAG12150.013.3991785
ATAACGC11900.013.3697483
GTACCGT1805.171387E-813.3611116
Produced by FastQC (version 0.11.5)