FastQCFastQC Report
Fri 29 Apr 2016
ERR1042533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915148
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89530.1513571596179842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.11367424788018829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63570.1074698384554368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37550.024.8801611
TCGTTTA8000.016.4187530
TAACGCC8750.016.0685714
ACGGACC12250.015.7061228
GTATCAA59450.015.5592942
AAGACGG14150.015.2968215
GTAAACG10200.015.23529427
GACGGAC12400.015.2177417
GTATTAG12500.015.0961
ATAACGC9750.014.9897433
TCTAGCG6100.014.86065628
CGCATCG9150.014.55737613
CGCAAGA13550.014.4723252
CAAGACG14750.014.1728814
AATAACG9900.014.0151522
CGCGTAT800.006302694313.87512
TAAACGC11350.013.85462628
AGACGGA13750.013.7236376
GCGCAAG14850.013.7037041
CGTTTAT9450.013.70370431
Produced by FastQC (version 0.11.5)