FastQCFastQC Report
Fri 29 Apr 2016
ERR1042532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8556890
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149600.17482987393784424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114300.13357656812229676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105480.12326908491285969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62800.024.0676751
CTAGCGG9300.016.51075229
TCGTTTA12750.016.3960830
ACGGACC19000.016.1631588
TCTAGCG9400.016.13829628
AAGACGG21200.015.9693395
GACGGAC19200.015.5130217
GTATCAA98450.015.2021332
CAAGACG22750.015.1252764
CGTTTAT13900.014.77338131
TAACGCC15400.014.5357144
ATAACGC15950.014.150473
CGCAAGA22650.013.9668872
CGGACCA22550.013.6186269
AGACGGA23150.013.5853136
TATACTG16500.013.4545465
CCGGTCG15050.013.3986720
TAGAGTG17000.013.1676475
TAATACT14650.013.1331064
TACGACG11750.013.0680855
Produced by FastQC (version 0.11.5)