FastQCFastQC Report
Fri 29 Apr 2016
ERR1042531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8301792
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111460.13426016937066118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.020.0256211
TCGTTTA13150.016.8821330
TCTAGCG11200.016.84821528
ACGGACC18850.016.7824948
GACGGAC18500.016.77
TAAACGC15550.016.41800728
GTAAACG15550.016.41800727
CTAGCGG11650.016.35622229
CGCAAGA21800.015.2752292
CAAGACG22400.015.1964294
TAACGCC15350.015.0651464
AGACGGA21900.014.9520546
CGCTTCG18200.014.94230732
CCGGTCG15000.014.92333220
AAGACGG22850.014.8971565
CGGACCA21600.014.817139
ATAACGC16800.014.5357143
CGCAATA13800.014.47826136
TTCGGGC18000.014.28611135
CGTTTAT15900.014.07861631
Produced by FastQC (version 0.11.5)