FastQCFastQC Report
Fri 29 Apr 2016
ERR1042529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13609689
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT247650.18196595087514492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT198700.1459989276757169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179090.1315900752765181No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT168740.12398519907398325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC40000.017.2512517
ACGGACC41150.016.9939258
AAGACGG43550.016.9919625
CAAGACG46150.016.8364034
CGGACCA43800.016.2191779
TCGTTTA29850.016.11390330
CGCAAGA45950.015.6213282
CTAGCGG23300.015.48283329
TCTAGCG22950.015.47712428
AGACGGA47100.015.318476
GCGCAAG47600.015.2741591
CGTTTAT32400.014.90277831
CGAACGA21550.014.85150716
ATGGTCG32150.014.38569336
TAACGCC35400.014.1624294
GGTATCA191900.014.0075561
ATAACGA23200.013.954741512
ACGAACG22850.013.92560115
CGCAATA26250.013.8838136
TAACGAA23100.013.85497913
Produced by FastQC (version 0.11.5)