FastQCFastQC Report
Fri 29 Apr 2016
ERR1042527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706399
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38770.22720360243999208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20250.11867095562057878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.026.7438581
GTATCAA17550.021.5042742
TAATACG555.1445E-420.1818184
CGCTATA500.007036243618.52
CGGATCG609.2393195E-418.537
TAACACT2150.018.0697674
ACCGTCC1156.4103006E-817.6956528
TCGCGTA5650.017.3539839
CGCGTAA5800.017.22413810
GTAATAC1401.87174E-917.1785723
GCTATAC1201.04204446E-716.9583323
GACGGAC8300.016.9397587
ACGGACC8550.016.6608188
TAGTACG1005.8811456E-616.6500024
GGTAATA1352.2213499E-816.4444452
GTCGCGT6000.016.3416678
GTATACT1251.6589729E-716.2799994
CAAGACG9100.016.2637354
CGCAAGA9000.016.238892
AGACGGA9100.016.0604386
Produced by FastQC (version 0.11.5)