FastQCFastQC Report
Fri 29 Apr 2016
ERR1042526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017537
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT35130.3452454308786806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27240.2677052529785158No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG19670.19330992386517643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.17414600157045887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.027.6681421
GTATCAA13550.022.937272
TACCGTC2100.022.023817
CGTCGTA1850.022.010
CCGTCGT1950.021.8205139
ATACCTA603.7257465E-521.5833326
ACGGACC5750.020.9130448
CGAACGA1950.020.87179616
TCGTTTA1251.8189894E-1120.7230
CGGACCA5900.020.0677979
ACCGTCG2150.019.7906978
GCGCAAG7000.019.2928581
GACGGAC6250.019.247
CGCAAGA6600.019.0606062
TCGTTCG4400.018.92045437
TAACGAA2100.018.513
TACCCCG500.00703434218.4999985
CAAGACG7050.018.3687934
GATAACG2250.018.08888811
GTCGTAG2400.017.72916611
Produced by FastQC (version 0.11.5)