FastQCFastQC Report
Fri 29 Apr 2016
ERR1042524.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042524.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679177
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17140.2523642585069871No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT13990.2059845960625875No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG13360.19670866357370762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.1437033350658223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC250.00549478129.5999983
CCCGTAG400.001930378323.1251
GGTATCA9700.021.7422681
TCGTTCG2650.021.6415137
GTATCAA11250.018.5822222
GTCTCGT3700.018.50000234
GTAACGG500.007031992518.4999983
TCTCGTT3800.018.01315935
GTACACA1258.560164E-917.764
GTAGAAC1054.7901267E-717.6190473
CTATACT752.0656978E-417.2666664
TCGTAAG650.001579034117.0769232
AGTACTA650.001579034117.0769235
GTAAACG650.001579034117.07692327
AGTCTCG4450.017.04494533
TCGCGTA4500.016.8555569
CTCGTTC3650.016.72602736
CGAAAAC1005.870672E-616.6523
CGCGTAA4850.016.40206110
GTTAGCA5000.016.27999920
Produced by FastQC (version 0.11.5)