FastQCFastQC Report
Fri 29 Apr 2016
ERR1042523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599943
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67700.1208940876719638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57490.10266175923576365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.020.522441
AAGACGG17600.016.6079545
CAAGACG19100.016.369114
ACGGACC17450.016.1146138
GACGGAC17500.016.0685717
CGCAAGA18400.015.5842382
TAACGAA9800.015.47959213
TATACCG1601.0992153E-815.031255
CGAACGA10100.015.01980116
CGGACCA18650.014.8793579
TCGTTTA10700.014.8691630
GCGCAAG21200.014.8349061
ATAACGA10700.014.52336512
GTATCAA61200.014.5098042
AGACGGA20500.014.2585366
TATACTG7300.014.1917815
ACGAACG10600.014.13679215
TACCGTC13100.014.1221387
TATAGTG7200.013.8749995
ATACTGT11200.013.5446426
Produced by FastQC (version 0.11.5)